Journal: The Plant Cell
Article Title: Two ARGONAUTE proteins loaded with transposon-derived small RNAs are associated with the reproductive cell lineage in Arabidopsis
doi: 10.1093/plcell/koad295
Figure Lengend Snippet: AGO5 and AGO9 expression throughout development. Expression of ProAGO5:EGFP-AGO5 (in cyan, A, C, E, G, I, K, M, O, Q, S, U, W) and ProAGO9:EGFP-AGO9 (in cyan, B, D, F, H, J, L, N, P, R, T, V, X) in plant lines containing the stem cell reporter ProCLV3:H2B- mCherry (red) and lacking endogenous AGO5 or AGO9 . A, B) Meristems of 7-day-old seedlings. Cc, Dd) Meristems of 14-d-old seedlings. E, F) Inflorescence meristems of 35-day-old plants. G) FB in stage 7; asterisk marks the upper region of the gynecium; arrowheads the inner layers of future anthers. H) as G) Arrowhead marks the base of the bud; asterisk the gynecium (asterisk); section signs (§) the inner layers of future stamens. I, J) Microspore mother cells in developing anthers. K, L) Mature pollen. M, N) megaspore mother cells (arrowhead) in developing ovules. O, P) Egg cells (arrowhead) within embryo sacs. Q, R) Zygotes (arrowhead). S, T) Embryos at the globular stage. U, V) Embryos at the heart stage. W, X) Meristem of 21-d-old plants grown under a short daylight regime. Scale bars k, l = 5 μ m; A, B, D, I, J, M, N, S, T, U, V, W, X = 20 μ m; C, E, F, G, H, O, P, Q, R = 50 μ m.
Article Snippet: Segmentation and counting of H2B-mCherry-labeled stem cell nuclei were computed with Imaris 9.5.0 software.
Techniques: Expressing